📖 The Scoop
The role of RNAPII in 3D genome organization has been heavily debated over the last two decades. Here, in situ Hi-C and Micro-C data were used to conclusively address this debate. I discovered that: 1) using cell population in situ Hi-C data, longer loops with enhanced signal appear upon RNAPII depletion. Together with a rewired-loop pattern of gained loops in the absence of RNAPII, I can suggest that RNAPII may impede cohesin extrusion in human cells; 2) using in situ Hi-C data in G1-sorted cells, I observed loops with longer length, stronger signal, and stronger insulation, which are in l...
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